Analysis Note

Positive ControlHepG2 cells

Application

Immunoblotting (1:2500)r>Immunocytochemistry (1:2500)r>Immunoprecipitation (1:250)r>

General description

TRB3 is a protein that is reported to disrupt insulin signaling by binding directly to Akt and blocking activation of the kinase.

This Anti-TRB3 (1-145) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of TRB3 (1-145).

Recognizes the ~45 kDa TRB3 protein in HepG2 cells.

Rabbit polyclonal antibdy supplied as undiluted serum that has been adsorbed against GST to remove GST-reactive antibodies. Recognizes the ~45 kDa TRB3 protein.

Immunogen

a recombinant protein consisting of amino acids 1-145 of mouse TRB3, fused to GST

Mouse

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Du, K., et al. 2003. Science300, 1574.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

For long-term storage aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)